Application

Detect using this mouse monoclonal Anti-Bin3, clone 3A4 Antibody, Cat. No. MABC1189-25UG, validated for use in Western Blotting.

Research CategoryApoptosis & Cancer

Western Blotting Analysis: 4 µg/mL from a representative lot detected Bin3 in 10 µg of MCF7 and HEK293 cell lysate.

Western Blotting Analysis: A representative lot detected Bin3 in Western Blotting applications (Ramalingam, A., et. al. (2008). Cancer Res. 68(6):1683-90).

Anti-Bin3, clone 3A4, Cat. No. MABC1189, is a mouse monocloanl antibody that detects Bridging integrator 3 (Bin3) protein and has been tested for use in Western Blotting.

Western Blotting Analysis: 4 µg/mL from a representative lot detected Bin3 in 10 µg of MCF7 and HEK293 cell lysate.
Western Blotting Analysis: A representative lot detected Bin3 in Western Blotting applications (Ramalingam, A., et. al. (2008). Cancer Res. 68(6):1683-90).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Bridging integrator 3 (UniProt: Q9NQY0; also known as Bin3) is encoded by the BIN3 gene (Gene ID: 55909) in human. Bin3 is a cytoskeletal protein that is ubiquitously expressed except in brain and is involved in cytokinesis and septation where it has a role in the localization of F-actin. It contain one BAR domain (aa 9-232), which forms coiled-coil structures. The BAR domain interacts with and facilitates tubulation of curved membranes and also binds to small GTPases and other cell regulatory proteins in the cytosol and nucleus. Deletion of Bin3 in mice is shown to cause cataracts associated with actin defects. Bin 3 I shown to suppress lymphoma during aging and restricts the efficiency of lung carcinogenesis. It is also shown to restrict the proliferation and motility of oncogenically transformed cells. (Ref.: Ramalingam, A., et al. (2008). Cancer Res. 68 (6): 1683-1690).

Immunogen

GST-tagged full length recombinant human Bridging integrator 3 (Bin3).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected Bin3 in 10 µg of HeLa cell lysate.

Specificity

Clone 3A4 detects Bridging integrator 3 (Bin3) in human and mouse cells.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~30 kDa observed; 29.66 kDa calculated. Uncharacterized bands may be observed in some lysate(s).